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Ion Channels "Methods and Protocols"
Every cell in our body contains a great variety and number of permeability pathways for various organic and inorganic ions, water, metabolites, nutrients, and signaling molecules. Maintenance and...
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Power failure: why small sample size undermines the reliability of neuroscience
A study with low statistical power has a reduced chance of detecting a true effect, but it is less well appreciated that low power also reduces the likelihood that a statistically significant result...
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[Protocols] In vitro imaging of primary neural cell culture from Drosophila
Cell culture systems are widely used for molecular, genetic and biochemical studies. Primary cell cultures of animal tissues offer the advantage that specific cell types can be studied in vitro outside...
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Small-scale isolation of synaptic vesicles from mammalian brain : Nature...
Synaptic vesicles (SVs) are essential organelles that participate in the release of neurotransmitters from a neuron. Biochemical analysis of purified SVs was instrumental in the identification of...
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Towards Neuronal Organoids: A Method for Long-Term Culturing of High-Density...
One of the goals in neuroscience is to obtain tractable laboratory cultures that closely recapitulate in vivo systems while still providing ease of use in the lab. Because neurons can exist in the body...
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A strategy to capture and characterize the synaptic transcriptome
Here we describe a strategy designed to identify RNAs that are actively transported to synapses during learning. Our approach is based on the characterization of RNA transport complexes carried by...
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Viral transduction of the neonatal brain delivers controllable genetic...
Freehand injection of recombinant AAV into the neonatal mouse brain offers a fast and easy way to attain widespread genetic manipulation of neurons throughout the brain. Rapid onset and year-long...
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Selective and regulated gene expression in murine Purkinje cells by in utero...
A new in utero electroporation (IUE) protocol has been developed to deliver genes preferentially into cerebellar Purkinje cells. IUE did not alter the physiological characteristics or normal synaptic...
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Localization of single-cell current sources based on extracellular potential...
A new, spike CSD (sCSD) method has been developed to reveal CSD distribution of single cells during action potential generation, based on the inverse solution of the Poisson-equation. Simulations...
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Researchers Perform Fastest Measurements Ever Made of Ion Channel Proteins
The miniaturization of electronics continues to create unprecedented capabilities in computer and communications applications, enabling handheld wireless devices with tremendous computing performance...
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Opportunities and challenges of pluripotent stem cell neurodegenerative...
Human neurodegenerative disorders are among the most difficult to study. In particular, the inability to readily obtain the faulty cell types most relevant to these diseases has impeded progress for...
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Seeing the forest tree by tree: super-resolution light microscopy meets the...
Light microscopy can be applied in vivo and can sample large tissue volumes, features crucial for the study of single neurons and neural circuits. However, light microscopy per se is...
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Targeting neurons and photons for optogenetics
activity in genetically or functionally defined neurons with millisecond precision. Harnessing the full potential of optogenetic tools, however, requires light to be targeted to the right neurons at...
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A chamber for the perfusion of in vitro tissue with multiple solutions
There are currently no practical systems that allow extended regions (>5 mm2) of a tissue slice in vitro to be exposed, in isolation, to changes in ionic conditions or to pharmacological...
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Contamination of current-clamp measurement of neuron capacitance by...
Measuring neuron capacitance is important for morphological description, conductance characterization, and neuron modeling. One method to estimate capacitance is to inject current pulses into a neuron...
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A new nonscanning confocal microscopy module for functional voltage-sensitive...
Recent advances in fluorescent confocal microscopy and voltage-sensitive and Ca2+ dyes have vastly improved our ability to image neuronal circuits. However, existing confocal systems are not fast...
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Method for stationarity-segmentation of spike train data with application to...
Correlations among neurons are supposed to play an important role in computation and information coding in the nervous system. Empirically, functional interactions between neurons are most commonly...
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Encoded multisite two-photon microscopy
The advent of scanning two-photon microscopy (2PM) has created a fertile new avenue for noninvasive investigation of brain activity in depth. One principal weakness of this method, however, lies with...
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Nanoscale-Targeted Patch-Clamp Recordings of Functional Presynaptic Ion Channels
Direct electrical access to presynaptic ion channels has hitherto been limited to large specialized terminals such as the calyx of Held or hippocampal mossy fiber bouton. The electrophysiology and...
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Idealizing Ion Channel Recordings by a Jump Segmentation Multiresolution Filter
Based on a combination of jump segmentation and statistical multiresolution analysis for dependent data, a new approach called J-SMURF to idealize ion channel recordings has been developed. It is...
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