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Viral transduction of the neonatal brain delivers controllable genetic...
Freehand injection of recombinant AAV into the neonatal mouse brain offers a fast and easy way to attain widespread genetic manipulation of neurons throughout the brain. Rapid onset and year-long...
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Selective and regulated gene expression in murine Purkinje cells by in utero...
A new in utero electroporation (IUE) protocol has been developed to deliver genes preferentially into cerebellar Purkinje cells. IUE did not alter the physiological characteristics or normal synaptic...
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Localization of single-cell current sources based on extracellular potential...
A new, spike CSD (sCSD) method has been developed to reveal CSD distribution of single cells during action potential generation, based on the inverse solution of the Poisson-equation. Simulations...
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Researchers Perform Fastest Measurements Ever Made of Ion Channel Proteins
The miniaturization of electronics continues to create unprecedented capabilities in computer and communications applications, enabling handheld wireless devices with tremendous computing performance...
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Opportunities and challenges of pluripotent stem cell neurodegenerative...
Human neurodegenerative disorders are among the most difficult to study. In particular, the inability to readily obtain the faulty cell types most relevant to these diseases has impeded progress for...
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Seeing the forest tree by tree: super-resolution light microscopy meets the...
Light microscopy can be applied in vivo and can sample large tissue volumes, features crucial for the study of single neurons and neural circuits. However, light microscopy per se is...
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Targeting neurons and photons for optogenetics
activity in genetically or functionally defined neurons with millisecond precision. Harnessing the full potential of optogenetic tools, however, requires light to be targeted to the right neurons at...
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A chamber for the perfusion of in vitro tissue with multiple solutions
There are currently no practical systems that allow extended regions (>5 mm2) of a tissue slice in vitro to be exposed, in isolation, to changes in ionic conditions or to pharmacological...
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Contamination of current-clamp measurement of neuron capacitance by...
Measuring neuron capacitance is important for morphological description, conductance characterization, and neuron modeling. One method to estimate capacitance is to inject current pulses into a neuron...
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A new nonscanning confocal microscopy module for functional voltage-sensitive...
Recent advances in fluorescent confocal microscopy and voltage-sensitive and Ca2+ dyes have vastly improved our ability to image neuronal circuits. However, existing confocal systems are not fast...
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Method for stationarity-segmentation of spike train data with application to...
Correlations among neurons are supposed to play an important role in computation and information coding in the nervous system. Empirically, functional interactions between neurons are most commonly...
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Encoded multisite two-photon microscopy
The advent of scanning two-photon microscopy (2PM) has created a fertile new avenue for noninvasive investigation of brain activity in depth. One principal weakness of this method, however, lies with...
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Nanoscale-Targeted Patch-Clamp Recordings of Functional Presynaptic Ion Channels
Direct electrical access to presynaptic ion channels has hitherto been limited to large specialized terminals such as the calyx of Held or hippocampal mossy fiber bouton. The electrophysiology and...
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Two-Photon Excitation STED Microscopy in Two Colors in Acute Brain Slices
Many cellular structures and organelles are too small to be properly resolved by conventional light microscopy. This is particularly true for dendritic spines and glial processes, which are very small,...
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Big Neuroscience: Billions and Billions (Maybe) to Unravel Mysteries of the...
The era of Big Neuroscience has arrived.In late January, The Human Brain Project—an attempt to create a computer simulation of the brain at every scale from the nano nano to the macro biotic—announced...
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Whole brain cellular-level activity mapping in a second
It is now possible to map the activity of nearly all the neurons in a vertebrate brain at cellular resolution. What does this mean for neuroscience research and projects like the Brain Activity Map...
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Neurient: An algorithm for automatic tracing of confluent neuronal images to...
We present Neurient, an algorithm developed to trace dense fluorescent neurite images.Neurient allows unsupervised image processing, with optional tunable parameters.Method: find seed points, index...
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See-through brains clarify connections
Technique to make tissue transparent offers three-dimensional view of neural networks.A chemical treatment that turns whole organs transparent offers a big boost to the field of ‘connectomics’ — the...
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An optogenetic approach in epilepsy
[Review] Highlights:Optogenetic tools allow selective activation/silencing of specific neuronal populations.Inhibitory opsins have been shown to efficiently reduce epileptiform activity in brain...
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Optical control of metabotropic glutamate receptors
G protein–coupled receptors (GPCRs), the largest family of membrane signaling proteins, respond to neurotransmitters, hormones and small environmental molecules. The neuronal function of many GPCRs has...
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