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Conversion of Channelrhodopsin into a Light-Gated Chloride Channel
The field of optogenetics uses channelrhodopsins (ChRs) for light-induced neuronal activation. However, optimized tools for cellular inhibition at moderate light levels are lacking. We found that...
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Single-cell axotomy of cultured hippocampal neurons integrated in neuronal...
An understanding of the molecular mechanisms of axon regeneration after injury is key for the development of potential therapies. Single-cell axotomy of dissociated neurons enables the study of the...
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The OpenPicoAmp : an open-source planar lipid bilayer amplifier for hands-on...
Neuroscience education can be promoted by the availability of low cost and engaging teaching materials. To address this issue, we developed an open-source lipid bilayer amplifier, the OpenPicoAmp,...
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High-fidelity optical reporting of neuronal electrical activity with an...
In this technical report, St-Pierre and colleagues introduce a new genetically encoded voltage sensor called Accelerated Sensor of Action Potentials 1 (ASAP1), which consists of a circularly permuted...
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All-optical electrophysiology in mammalian neurons using engineered microbial...
A combination of a sensitive blue light-gated channelrhodopsin actuator and red-shifted Arch-based voltage sensors allows all-optical electrophysiology without cross-talk in cultured neurons or brain...
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Ultrafast tissue staining with chemical tags
Cellular and subcellular structures in thick biological samples typically are visualized either by genetically encoded fluorescent proteins or by antibody staining against proteins of interest....
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Des conduits de nerfs imprimés par micro-stéréolithographie
Des scientifiques de l'université de sheffield, ont mis au point un procédé d'impression 3D pour fabriquer des conduits de guidage pour réparer des nerfs.See it on Scoop.it, via Neuroscience_technics
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StimDuino: An Arduino-based electrophysiological stimulus isolator
Highlights- Cheap, microcontroller-based, programmable electrophysiological stimulus isolator.- Allows automatic control over stimulation parameters.- Multi-purpose application for in vivo and in vitro...
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