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Neurient: An algorithm for automatic tracing of confluent neuronal images to determine alignment

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  • We present Neurient, an algorithm developed to trace dense fluorescent neurite images.
  • Neurient allows unsupervised image processing, with optional tunable parameters.
  • Method: find seed points, index into a directed lookup table, trace neurite segments.
  • Trace coordinates and orientations can be used to evaluate neurite alignment. 
  • The Neurient code is open source and freely available for use and customization.

by Mitchel JAet al., Journal of Neuroscience MethodsVolume 214, Issue 2, 15 April 2013, Pages 210–222




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